| VZV-gE

Varicella-zoster virus (VZV) glycoprotein E (gE), encoded by ORF68, is the most abundant viral glycoprotein. Deletion of ORF68 is incompatible with the recovery of infectious virus from VZV cosmids. Varicella-zoster virus gE is essential, as demonstrated by the restoration of infectivity following non-natural site substitution of ORF68 in the genome, and further evidenced by the role of gI in promoting the maturation and intracellular distribution of this critical varicella-zoster virus glycoprotein.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	50	25	12.5	6.25	3.12	1.56	0.78	0
OD	2.44	1.44	0.94	0.61	0.35	0.23	0.16	0.08
Corrected OD	2.36	1.36	0.86	0.53	0.27	0.15	0.08	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse VZV-gE spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	83-111	93
Plasma(n=8)	85-105	97
Cell Culture Supernates(n=8)	83-112	96

 

| Linearity

Samples were spiked with high concentrations of Mouse VZV-gE and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-115	88-106	83-111
	Average (%)	99	94	97
1 : 4	Range (%)	90-111	84-99	86-113
	Average (%)	104	92	103